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PpERF9 interacts with PpTPL1. A) Interaction between PpERF9 and PpTPL1 in a yeast two-hybrid assay, with pGADT7-T and <t>pGBKT7-53</t> as positive controls. DDO, SD/−Trp/−Leu medium; QDO/X/A, SD/−Trp/−Leu/−Ade/−His medium with X-α-gal and Aureobasidin A. B) Subcellular localization of PpTPL1 in N. benthamiana leaf cells. C) Physical association between PpTPL1 and PpERF9 confirmed by a BiFC assay. Bars = 25 µm. D) Schematic diagram of the domain structures of the PpERF9 protein in the wild-type control and in the generated mutants. E) Yeast two-hybrid assays indicated that PpTPL1 interacts with either EAR motif in PpERF9. A mutation to only 1 of the EAR motifs in PpERF9 did not affect the interaction between PpERF9 and PpTPL1. However, the interaction was eliminated when both EAR motifs were mutated. F) Schematic diagram of the domain structures of the PpTPL1 protein in the wild-type control and in the generated mutants. Yeast two-hybrid assays indicated that PpERF9 interacts with the CTLH domain of PpTPL1.
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PpERF9 interacts with PpTPL1. A) Interaction between PpERF9 and PpTPL1 in a yeast two-hybrid assay, with pGADT7-T and <t>pGBKT7-53</t> as positive controls. DDO, SD/−Trp/−Leu medium; QDO/X/A, SD/−Trp/−Leu/−Ade/−His medium with X-α-gal and Aureobasidin A. B) Subcellular localization of PpTPL1 in N. benthamiana leaf cells. C) Physical association between PpTPL1 and PpERF9 confirmed by a BiFC assay. Bars = 25 µm. D) Schematic diagram of the domain structures of the PpERF9 protein in the wild-type control and in the generated mutants. E) Yeast two-hybrid assays indicated that PpTPL1 interacts with either EAR motif in PpERF9. A mutation to only 1 of the EAR motifs in PpERF9 did not affect the interaction between PpERF9 and PpTPL1. However, the interaction was eliminated when both EAR motifs were mutated. F) Schematic diagram of the domain structures of the PpTPL1 protein in the wild-type control and in the generated mutants. Yeast two-hybrid assays indicated that PpERF9 interacts with the CTLH domain of PpTPL1.
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Summary of the activities and input line items considered in the costing analysis covering the 2015–2017 period in Senegal.
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PpERF9 interacts with PpTPL1. A) Interaction between PpERF9 and PpTPL1 in a yeast two-hybrid assay, with pGADT7-T and pGBKT7-53 as positive controls. DDO, SD/−Trp/−Leu medium; QDO/X/A, SD/−Trp/−Leu/−Ade/−His medium with X-α-gal and Aureobasidin A. B) Subcellular localization of PpTPL1 in N. benthamiana leaf cells. C) Physical association between PpTPL1 and PpERF9 confirmed by a BiFC assay. Bars = 25 µm. D) Schematic diagram of the domain structures of the PpERF9 protein in the wild-type control and in the generated mutants. E) Yeast two-hybrid assays indicated that PpTPL1 interacts with either EAR motif in PpERF9. A mutation to only 1 of the EAR motifs in PpERF9 did not affect the interaction between PpERF9 and PpTPL1. However, the interaction was eliminated when both EAR motifs were mutated. F) Schematic diagram of the domain structures of the PpTPL1 protein in the wild-type control and in the generated mutants. Yeast two-hybrid assays indicated that PpERF9 interacts with the CTLH domain of PpTPL1.

Journal: The Plant Cell

Article Title: The ethylene-responsive transcription factor PpERF9 represses PpRAP2.4 and PpMYB114 via histone deacetylation to inhibit anthocyanin biosynthesis in pear

doi: 10.1093/plcell/koad077

Figure Lengend Snippet: PpERF9 interacts with PpTPL1. A) Interaction between PpERF9 and PpTPL1 in a yeast two-hybrid assay, with pGADT7-T and pGBKT7-53 as positive controls. DDO, SD/−Trp/−Leu medium; QDO/X/A, SD/−Trp/−Leu/−Ade/−His medium with X-α-gal and Aureobasidin A. B) Subcellular localization of PpTPL1 in N. benthamiana leaf cells. C) Physical association between PpTPL1 and PpERF9 confirmed by a BiFC assay. Bars = 25 µm. D) Schematic diagram of the domain structures of the PpERF9 protein in the wild-type control and in the generated mutants. E) Yeast two-hybrid assays indicated that PpTPL1 interacts with either EAR motif in PpERF9. A mutation to only 1 of the EAR motifs in PpERF9 did not affect the interaction between PpERF9 and PpTPL1. However, the interaction was eliminated when both EAR motifs were mutated. F) Schematic diagram of the domain structures of the PpTPL1 protein in the wild-type control and in the generated mutants. Yeast two-hybrid assays indicated that PpERF9 interacts with the CTLH domain of PpTPL1.

Article Snippet: Briefly, the CDS of each gene ( PpERF9 and PpRAP2.4 ) was fused with the VP16 fragment and inserted into the pGBKT7 (BD) vector (gene-VP16-BD).

Techniques: Y2H Assay, Bimolecular Fluorescence Complementation Assay, Generated, Mutagenesis

Journal: Frontiers in Molecular Biosciences

Article Title: SARS-CoV-2 Spike Protein Extrapolation for COVID Diagnosis and Vaccine Development

doi: 10.3389/fmolb.2021.607886

Figure Lengend Snippet: Molecular diagnostic assays used for the diagnosis of COVID-19.

Article Snippet: SARS-CoV-2 S gene for BD Max , Real-time RT-PCR , Becton Dickinson Surgical Industries, LTD. , Nasopharyngeal and oropharyngeal swabs , S gene , Results for up to 24 samples in <3 h , Brazil.

Techniques: Diagnostic Assay, Quantitative RT-PCR, RNA Extraction, Multiplex Assay, Real-time Polymerase Chain Reaction, Detection Assay, Microarray

Summary of the activities and input line items considered in the costing analysis covering the 2015–2017 period in Senegal.

Journal: Vaccines

Article Title: The Costs of Introducing the Hepatitis B Birth Dose Vaccine into the National Immunization Programme in Senegal (NéoVac Study)

doi: 10.3390/vaccines9050521

Figure Lengend Snippet: Summary of the activities and input line items considered in the costing analysis covering the 2015–2017 period in Senegal.

Article Snippet: The purchase of monovalent HepB-BD vaccines (10-dose vials; Hepavax-Gene ® , Berna Biotech Korea Corp., Korea) was overseen by the Prevention Department of the Ministry of Health.

Techniques: Activity Assay, Injection

Main data used in the costing analysis of  hepatitis B birth dose  introduction in Senegal.

Journal: Vaccines

Article Title: The Costs of Introducing the Hepatitis B Birth Dose Vaccine into the National Immunization Programme in Senegal (NéoVac Study)

doi: 10.3390/vaccines9050521

Figure Lengend Snippet: Main data used in the costing analysis of hepatitis B birth dose introduction in Senegal.

Article Snippet: The purchase of monovalent HepB-BD vaccines (10-dose vials; Hepavax-Gene ® , Berna Biotech Korea Corp., Korea) was overseen by the Prevention Department of the Ministry of Health.

Techniques:

Aggregation method for calculating costs of HepB-BD implementation in Senegal. Source: Adapted from Brenzel L. Common approach for the costing and financing analyses of routine immunization and new vaccine introduction costs (NUVI). Bill and Melinda Gates Foundation; 2013.

Journal: Vaccines

Article Title: The Costs of Introducing the Hepatitis B Birth Dose Vaccine into the National Immunization Programme in Senegal (NéoVac Study)

doi: 10.3390/vaccines9050521

Figure Lengend Snippet: Aggregation method for calculating costs of HepB-BD implementation in Senegal. Source: Adapted from Brenzel L. Common approach for the costing and financing analyses of routine immunization and new vaccine introduction costs (NUVI). Bill and Melinda Gates Foundation; 2013.

Article Snippet: The purchase of monovalent HepB-BD vaccines (10-dose vials; Hepavax-Gene ® , Berna Biotech Korea Corp., Korea) was overseen by the Prevention Department of the Ministry of Health.

Techniques:

Economic and financial costs (in USD 2016) per  HepB-BD  vaccinated newborn in Senegal.

Journal: Vaccines

Article Title: The Costs of Introducing the Hepatitis B Birth Dose Vaccine into the National Immunization Programme in Senegal (NéoVac Study)

doi: 10.3390/vaccines9050521

Figure Lengend Snippet: Economic and financial costs (in USD 2016) per HepB-BD vaccinated newborn in Senegal.

Article Snippet: The purchase of monovalent HepB-BD vaccines (10-dose vials; Hepavax-Gene ® , Berna Biotech Korea Corp., Korea) was overseen by the Prevention Department of the Ministry of Health.

Techniques: